Hepg2 dual
WebTo investigate whether the enhanced cytotoxicity of chemicals affects DNA damage and DNA repair capability simultaneously, a dual luciferase assay was conducted in which CdCl 2-damaged reporter plasmid and gadd153-luc plasmid were transiently transfected into HepG2 cells. Table II showed that using dual luciferase assay could detect the DNA ... Web5 apr 2024 · In this study, the novel multifunctional liposomes (CAPS-CUR/GA&Gal-Lip) were prepared for co-delivery of curcumin (CUR) and capsaicin (CAPS), in which glycyrrhetinic acid (GA) and galactose (Gal) were chosen as targeting ligands to modify the liposomes (Lip) for dual-targeting liver cancer.
Hepg2 dual
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WebThen, the 96-well plate was shaken on a swing bed for 10 min at 37°C. Finally, the absorbance of the solution in each well was measured with a microplate reader (TECAN NanoQuant plate Infinite 200 Pro) at 570 nm to evaluate the viability of the HepG2 cells. Ability of the in vitro Dual-Modal Imaging WebIntroduction. Cisplatin, cis-diamminedichloroplatinum (II) (CDDP), is a chemotherapy drug used in the treatment of solid tumors [1, 2].It exerts biological activity when it enters the cell and subsequently binds to DNA [] but cisplatin shows significant dose-related side effects such as nephrotoxicity, neurotoxicity, nausea and vomiting [].In order to maintain the …
Web19 set 2024 · Briefly, HepG2 cells or DGKθ-T2A-Luciferase knock-in HepG2 cells were plated on a 24-well plate at a density of 2 × 10 5 cells per well for 24 h followed by the treatment with Bt 2 cAMP ... the cells were harvested and the transcriptional activity of the hDGKθ reporter gene was measured using a dual luciferase assay kit (Promega). Web4 dic 2024 · HepG2 is a popular hepatic cell line. It is used in a wide range of studies, from the oncogenesis to the cytotoxicity of substances on the liver. In this regard, we set out …
WebSaroglitazar, a dual PPARα/γ agonist with predominant PPARα activity, shows an overall improvement in NASH. ... NFkB phosphorylation and stellate cell activation in HepG2 … WebAl contatore di cellule: Cellule HepG2. Le cellule HepG2 sono una linea cellulare derivata da tessuto epatico di un paziente con carcinoma epatocellulare (Hepatocellular Carcinoma, …
HepG2-Dual™ cells are designed for the study of NF-κB and IRF (interferon regulatory factor) pathways. NF-κB activation can be assessed by measuring secreted embryonic alkaline phosphatase (SEAP) activity, while the IRF pathway can be monitored by following Lucia luciferase activity.
Web4 ago 2016 · The harmful effects of bile acid accumulation occurring during cholestatic liver diseases have been associated with oxidative stress increase and endothelial nitric oxide synthase (NOS-3) expression decrease in liver cells. We have previously reported that glycochenodeoxycholic acid (GCDCA) down-regulates gene expression by increasing … first choice primor credit cardWeb26 mag 2014 · The objective of this study is to investigate the contributing effect of the nuclear transcription factor-erythroid 2-related factor 2 (Nrf2)-mediated signaling pathway on the indirect antioxidant capacity of caffeic acid phenethyl ester (CAPE) against oxidative stress in HepG2 cells. The result of an antioxidant response element (ARE)-luciferase … evan rachel wood frozen 2 behind the scenesWebIn the present study, it was shown that IL-18 and its receptors (IL-18R) were constitutively expressed in hepatoma cell lines HepG2 and HepG2.2.15 as well as normal liver cell line HL-7702. We demonstrated that IL-18 directly inhibited HBV replication in HepG2.2.15 cells via downregulating the activities of HBV core and X gene promoters. evan rachel wood house of cardsWebHepG2 cells were treated by 200 μM H2O2 with or without a series of concentrations of NAS ... which is confirmed by the appearance of apoptotic bodies with PI and AO/EB dual staining, ... first choice primary care sebring flWebA dual near-infrared pH fluorescent probe has been designed, synthesized and applied to HepG2 cells, with a pKa value of 5.14 under acidic conditions and 11.31 under basic conditions, which is valuable for studying acidic organelles in living cells and pH changes in chemical systems. first choice primary care macon ga fax numberWeb15 feb 2024 · Monitoring the fluctuation of H 2 S in insulin-resistant HepG2 cells and diabetic mice with a dual-locked NIR fluorescent probe. Author links open overlay panel Zimeng … evan rachel wood marilyn manson redditWeb11 apr 2024 · Human HCC cell lines MHCC-97H, HepG2, and HEK293T were obtained from the American Type Culture Collection. ... In this study, we identified this dual role of miR-3180 for the first time. First, miR-3180 expression correlated negatively with CD36 and SCD1 expression in patients with HCC. first choice prior auth